When to Test After Day 3-7 Embryo Transfers

Timing of when you can reliably complete a pregnancy test for embryo transfer depends on the type of transfer: a day 3, day 5, or day 7 embryo.

Each type has a different timeline for development and implantation, which impacts when hCG (the pregnancy hormone) is likely to be detectable.

By testing at the right time—whether you had a Day 3, 5, or 7 transfer—you can ensure accurate results and avoid unnecessary stress.

Below, we’ll break down each embryo transfer type and offer guidelines for when to take a pregnancy test.

Learn more about pregnancy as a surrogate here, or fill out this simple form to get help if you are considering becoming a surrogate.

How Many Days After Embryo Transfer Can You Test?

Most fertility experts recommend waiting 10-14 days after any embryo transfer to take a pregnancy test. This time frame allows hCG levels to reach detectable levels.

Is It Too Early to Test 7 Days After Embryo Transfer?

Yes, testing 7 days after embryo transfer is generally too early for most people. Waiting until at least 9-10 days post-transfer for blastocyst (5- or 7-day) embryos or 12-14 days for 3-day embryos provides a more reliable result.

The earliest a positive pregnancy test can be detected is typically 9-10 days post-transfer for 5- and 7-day embryos, while 3-day transfers may require up to 12-14 days.

Testing earlier increases the risk of a false negative, as hCG levels may still be below the detection threshold.

Here’s a detailed look at when to test based on the specific embryo transfer day.

3-Day Embryo Transfer Pregnancy Tests

What is a 3-Day Embryo Transfer?

In a 3-day embryo transfer, the embryo has been allowed to develop for three days post-fertilization before being transferred to the uterus. At this stage, the embryo is usually a 6-8 cell structure.

When Does Implantation Occur?

Implantation after a 3-day embryo transfer typically happens 5-7 days post-transfer, meaning it may implant on days 8-10 after fertilization.

How Soon to Test for Pregnancy After a 3-Day Embryo Transfer

Because implantation can occur several days post-transfer, it’s best to wait until 12-14 days after a 3-day transfer to take a pregnancy test. Testing earlier may result in a false negative because hCG levels may not yet be detectable.

Signs and Symptoms to Watch For

During the waiting period, you might experience mild symptoms such as cramping, light spotting, or breast tenderness. However, these signs can also result from hormonal changes and are not definitive signs of pregnancy.

5-Day Embryo Transfer Pregnancy Tests

What is a 5-Day Embryo Transfer?

A 5-day embryo transfer involves transferring a blastocyst, a more advanced embryo stage with hundreds of cells, ready to implant. Blastocysts have a higher chance of implantation compared to 3-day embryos.

When Does Implantation Occur?

With a 5-day embryo, implantation usually occurs within 1-3 days post-transfer. Since these embryos are further along in development, they tend to implant sooner.

How Soon to Test for Pregnancy After a 5-Day Embryo Transfer

Since implantation may happen sooner, you may get an accurate result as early as 9-10 days after a 5-day transfer. However, to avoid potential false negatives, waiting until 10-12 days post-transfer is generally recommended.

Signs and Symptoms to Watch For

Similar to a 3-day transfer, you may experience cramping or spotting as implantation occurs. Other symptoms can include bloating, nausea, or fatigue. However, these signs are not conclusive and can be affected by medications used during the IVF process.

7-Day Embryo Transfer Pregnancy Test

What is a 7-Day Embryo Transfer?

A 7-day embryo transfer involves an embryo that has been developed for a full week before transfer. These embryos are at an advanced blastocyst stage and are often highly viable.

When Does Implantation Occur?

Implantation for a 7-day embryo may happen quite quickly, potentially within 1-2 days after transfer.

How Soon Can I Test for Pregnancy After a 7-Day Embryo Transfer

Since implantation generally occurs shortly after transfer, you could consider testing 9-10 days after a 7-day transfer. However, waiting for 10-12 days post-transfer provides the most reliable result.

Signs and Symptoms to Watch For

After a 7-day embryo transfer, signs like cramping or mild spotting may appear sooner than with earlier-stage transfers. Again, any symptoms should be taken with caution, as they could be due to other factors.

Get Support Today

Testing too soon can lead to unnecessary stress and potentially inaccurate results, so you should always follow your medical provider’s guidance on when to test. They will provide the best advice on when to test based on your transfer type and treatment plan.

Learn more about pregnancy as a surrogate here, or fill out this simple form to get help if you are considering becoming a surrogate.